Product Information

• CFX Connect™ Real-Time PCR Detection System
• Brand:
• Catalog No.: 185-5200

Product Description

The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch™ Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.

The CFX Connect Real-Time PCR Detection System offers: Easy startup - obtain great results right away with factory-calibrated dyes, quick setup, and intuitive software Effortless optimization - save time and reduce costs by optimizing assays in a single run using the thermal gradient Powerful data analysis tools - quickly and accurately validate and analyze data with the advanced analysis modules of CFX Maestro™ Software Accelerated publication submission - include MIQE annotations and generate RDML files using Biogazelle's qbase+ Software

Innovative Optical Design The CFX Connect™ system's solid-state optical technology (three filtered LEDs and three filtered photodiodes) provides sensitive detection for precise quantification and target discrimination. Scanning just above the sample plate, the optics shuttle individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk. The optical system automatically collects data from all wells during data acquisition, so you can enter or edit well information on your own schedule

Two-Target Multiplexing The CFX Connect system can discriminate up to two targets in a single reaction well. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels. At every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always fixed and optimal, and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference dye.

Fluorescence Resonance Energy Transfer (FRET) The CFX Connect system's optics shuttle includes one channel with an LED-filter photodiode combination dedicated for FRET singleplex experiments, further expanding your experimental options.

Efficient Optimization Determining the optimal temperature for primer annealing is crucial for efficient and specific amplification of product. With the CFX Connect™ system's thermal gradient feature, you can determine the optimal temperature for primer annealing in a single experiment, minimizing the use of precious samples and reagents and saving valuable research time. At any step in a protocol, you can program a temperature gradient of up to 24°C across the reaction block. The thermal cycler provides exceptional temperature uniformity and reproducibility within each gradient zone, and the temperatures can easily be programmed and viewed onscreen in the software so you can quickly identify the optimal incubation temperature.

Single tube loading for stat samples

Temperature-controlled sample loading for sensitive samples

Sample agitation on board to keep cells in suspension