The iScript reverse transcription supermix for RT-qPCR is a simple, fast, and sensitive first-strand cDNA synthesis kit for gene expression analysis using real-time qPCR. In one tube, the preblended 5x supermix contains all the necessary components except RNA template for reverse transcription.



1. Increase reproducibility of reverse transcription and reduce errors during setup — 1-tube format for simple and fast setup and reduced pipetting variability
2. Detect low-level target genes and conserve RNA during gene expression analysis — broad linear dynamic range of total input RNA (1 µg–1 pg) with a highly efficient RNase H+ MMLV reverse transcriptase
3. Use with dilute RNA samples — 5x concentration of supermix allows for greater volume of RNA sample in a 20 µl cDNA synthesis reaction
4. Eliminate freeze/thaw cycles — liquid format at –20ºC shortens overall processing time and maximizes stability
5. Validate purity of input RNA and gene expression results — optional no-RT controls for detecting genomic DNA contamination
6. Obtain accurate results — potent blend of RNase inhibitor prevents RNA degradation during reaction setup and reverse transcription
7. Increase primer design flexibility and prevent 5' and 3' bias — optimum blend of oligo(dT) and random primers in the 5x supermix for complete RNA coverage
8. Complete cDNA synthesis and qPCR the same day — fast 26 minute cDNA synthesis protocol