Product Description

The Comparative Proteomics Kit II: Western Blot Module is the second part of a two-part laboratory. In the first part of this laboratory (Comparative Proteomics Kit I: Protein Profiler Module), students use sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) to generate protein profiles and to visualize the unique array of proteins comprising muscle tissues from different fish. In this second part of the laboratory, students apply western blotting techniques to their polyacrylamide gel results to specifically identify myosin light chain from the hundreds of proteins that comprise the muscle cell extracts of closely and distantly related species of fish.

1. Applies immunology

1. Teaches students to use antibodies as detection tools

1. Serves as a laboratory extension for the Comparative Proteomics I Kit: Protein Profiler Module